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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Mol Cell. 2019 Sep 4;75(6):1218–1228.e6. doi: 10.1016/j.molcel.2019.07.027

Figure 4. Analysis of decay kinetics for select basal mRNAs during 2–5AMD.

Figure 4.

(A) Design of qPCR for detection of full-length mRNAs. (B) Decay of select highly expressed housekeeping mRNAs upon poly I:C treatment of A549 cells measured by qPCR. The decay of SON and PKRDC shows distinctly biphasic character indicating the presence of non-cleaved mRNA fraction (2–5AMD-resistant pool) in the cells. The inset graphs show log-linear parts of the decay profiles used to measure the first-order decay kinetic parameters. Dotted lines mark approximate levels of 2–5AMD-resistant mRNA fractions. Data are aggregates from three biological replicates. (C) Decay half-life (T1/2) obtained for each mRNA from the qPCR in (B). The last line shows T1/2 for 5,000 mRNAs calculated based on RNA-seq (Fig S4B, Table S3). (*) Excluding GAPDH due to its stability.