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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Mol Cell. 2019 Sep 4;75(6):1218–1228.e6. doi: 10.1016/j.molcel.2019.07.027

Figure 5. RNA-seq profiling of mRNA decay by RNase L in live cells and in cytosolic extracts.

Figure 5.

(A) Time-dependent decay of the most abundant 5,000 mRNAs (~90% of the mRNA pool) measured by spike-in RNA-seq. To obtain mRNAs levels, total reads for each sample were normalized using D. melanogaster RNA spike in as an internal standard. Transcripts are ordered from the highest to the lowest expression level in the untreated sample. (B) RNA-seq profiles for select individual mRNAs from (A). (C) Cleavage profiles and kinetic parameters for the mRNAs in (B) obtained in cell-free experiments (Rath et al., 2015). See also Table S3.