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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Mol Cell. 2019 Sep 4;75(6):1218–1228.e6. doi: 10.1016/j.molcel.2019.07.027

Figure 6. Poly-A+ transcriptome composition and dynamics during 2–5AMD.

Figure 6.

(A) Time evolution of the poly-A+ transcriptome obtained from spike-in RNA-seq. Induced transcripts (red) were upregulated by ≥ 5-fold at four hours of poly I:C treatment. (B) Illustration of read increase for a gene that is induced. (C) Levels of select basal and defense mRNAs measured by spike-in RNAs-seq. (D) Induction of innate immune mRNAs in response to poly I:C with and without 2–5AMD. (E) Experimental outline for measurement of innate immune mRNA decay. Innate immune mRNAs are allowed to accumulate followed by transcriptional inhibition with actinomycin D and decay profiling. (F) Kinetics parameters of defense mRNAs measured as in Fig. 4B. P-value compares T1/2 for defense vs basal mRNAs in Fig. 4C. See also Table S3.