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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Mol Cell. 2019 Sep 4;75(6):1218–1228.e6. doi: 10.1016/j.molcel.2019.07.027

Figure 7. Proposed model for coordination between transcription and decay in 2–5AMD.

Figure 7.

(A) A kinetic scheme for 2–5AMD and the IFN response with positive feedback. Parameters I (IFN mRNAs), A (2–5AMD-resistant stable activator within positive feedback), α and β are described in “Decay and transcriptional dynamics analysis section” (Methods). (B) Modeled dynamics of basal and defense mRNAs when all transcripts have the same sensitivity to 2–5AMD. Defense mRNAs are induced with positive feedback. (C) Model in (B) modified to include a stable activator of positive feedback. (D) Model in (B) modified to include both, a stable activator and stability of defense mRNAs. The modeling analysis in panels A-C is described in Methods. The slope difference for solid red lines in Fig. 7D vs 7C reflects the contribution of longer half-life to the overall decay rate. (E) The proposed model for mRNA decay and IFN transcriptional response leading to RNase L-mediated reorganization global translation. See also Figure S7.