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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Cell. 2019 Sep 5;179(1):74–89.e10. doi: 10.1016/j.cell.2019.08.001

Figure 1. NCCs, but not macrophages, colonize the trunk region during early development.

Figure 1.

(A) Trunk lateral views of Tg(olig2:DsRed);Tg(mpeg1:GFP) embryos at 24, 36 and 48 hpf. Arrowheads denote mpeg1+ macrophages. (B) Macrophage quantification in a 0.27 mm2 region in the trunk of embryos at 24, 36 and 48 hpf (mean ± SD). (C) 2D distribution of macrophages in the trunk of embryos at 24, 36 and 48 hpf (n = 9 fish in each condition). Diagram on the left shows the region quantified. All images used for quantification are aligned based on the position of the ventral edge of the spinal cord (dashed line). (D) Lateral view of a Tg(sox10:TagRFP);Tg(bactin2:Gal4);Tg(UAS:secA5-YFP) embryo at 20 hpf. Blue shading denotes dorsal trunk. Filled arrowheads denote dead cells in the dorsal trunk. Open arrowheads denote debris in the ventral trunk/yolk extension. (E) Quantification of the percentage of somites with secA5+ cells in 20 and 36 hpf embryos (n = 6/9 fish for 20/36 hpf). Dashed lines mark the ventral edge of the spinal cord. Scale bars, 50 μm.