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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Cell. 2019 Sep 5;179(1):74–89.e10. doi: 10.1016/j.cell.2019.08.001

Figure 4. The formation of PI(3)P+ and Lamp1+ phagosomes after NCC engulfment.

Figure 4.

(A) Images from a time-lapse movie starting at 19 hpf in a Tg(sox10:nls-Eos) embryo treated with neutral red at 16 hpf. Arrows denote a NCC engulfment vesicle that was gradually stained by neural red. (B) Images from a time-lapse movie of a 24 hpf Tg(sox10:Gal4);Tg(UAS:GFP-FYVE) embryo showing the fusion of scattered PI(3)P signals (arrowheads) with an engulfment vesicle (arrows). Schematic diagrams are shown below. (C) Images of an engulfment vesicle from a time-lapse movie of a 28 hpf Tg(sox10:Gal4);Tg(UAS:GFP-FYVE) embryo treated with neutral red. (D) Quantification of PI(3)P+ vesicle formation and normalized neutral red brightness over time (mean ± SD, n = 6 vesicles). Dashed line indicates time points with a small sample size. (E) Images from a time-lapse movie of a Tg(sox10:TagRFP);Tg(sox10:lamp1-GFP) embryo starting at 20 hpf. Arrowheads denote gradual enrichment of Lamp1-GFP in a NCC engulfment vesicle. (F) Orthogonal views of a Lamp1-GFP+ vesicle in a NCC. Scale bars, 10 μm in A, B, E and F; 2 μm in C.