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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Cell. 2019 Sep 5;179(1):74–89.e10. doi: 10.1016/j.cell.2019.08.001

Figure 5. Laser ablation-induced NCC phagocytosis.

Figure 5.

(A) Images from a time-lapse movie of a Tg(sox10:Gal4);Tg(UAS:GFP-FYVE) embryo after ablation of a NCC (outlined in yellow) at 24 hpf. Arrowheads denote NCC engulfment vesicles. Cyan and red arrowheads denote newly-formed and pre-existing vesicles, respectively, in each frame, correlating with the quantification in B. A NCC that engulfed twice is outlined in cyan. (B) Quantification of phagosome formation in panel A. Top panel shows phagosome count per frame fitted with a Gaussian curve (R square = 0.7075). Bottom panel shows the duration of each PI(3)P+ vesicle. (C) Quantification of the number of times NCCs phagocytose in a 100 μm2 region around the ablation site in 100 mpa (mean ± SD, n = 5 fish). (D & E) Quantification of the number of NCC phagosomes per frame before and after cell ablation (D), or distal and proximal to ablation sites (E) (n = 50 frames). mpa, minutes post ablation. Scale bar, 10 μm.