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. Author manuscript; available in PMC: 2020 Mar 2.
Published in final edited form as: Nat Immunol. 2019 Sep 2;20(10):1393–1403. doi: 10.1038/s41590-019-0468-0

Figure 7. CXCR4 signaling is necessary for Igk recombination.

Figure 7.

a, Expression (RNA-Seq) of all Vks, Jks and Ck genes in WT and mb1-Cre+/−Cxcr4fl/fl (Cxcr4−/−) small pre-B cells (n=2). b, Expression (RNA-Seq) of all Vks, Jks and Ck genes in WT pre-B cells cultured as indicated (n=2). c, Semi-quantitative PCR analysis of Igk rearrangements in WT and Cxcr4−/− small pre-B cells. Data is representative of three experiments. d, Quantitative analysis of Vk-Jk rearrangements in WT and Cxcr4−/− small pre-B cells compared to WT immature B cells. (n=4). Data are presented as mean±SD. P values were determined by unpaired t-test. *P<0.001 versus WT small pre-B cells. e, Semi-quantitative PCR analysis of Igk rearrangements in cultured WT pre-B cells. Data is representative of three experiments. f, Surface IgM+ B cells after culture of WT pre-B cells as indicated. Data is representative of four experiments. g, Histogram of Igκ expression pre-B cells cultured as indicated (n=3). h,i, Quantified and integrated transcription across all Vk genes segments in in vivo B cell progenitors (h) and in vitro cultured pre-B cells (i) including immediately before and after Vk gene bodies.