| Antibodies |
| Sheep anti-DIG-AP, Fab fragments |
Roche |
Cat# 11093274910, RRID:AB_2734716 |
| Sheep anti-Fluorescein-AP, Fab fragments |
Roche |
Cat# 11426338910 |
| Bacterial and Virus Strains |
| NEB 5-alpha competent E.coli
|
NEB |
Cat# C2987I |
| Chemicals, Peptides, and Recombinant Proteins |
| 5’ lacZ Atto 565 smFISH probe |
Shawn Little’s lab |
Little et al., 2013 |
| 3’ lacZ Atto 633 smFISH probe |
Shawn Little’s lab |
Little et al., 2013 |
|
hb Atto 565 smFISH probe |
Shawn Little’s lab |
Little et al., 2013 |
|
lacZ ISH DIG RNA probe |
This lab |
N/A |
|
hb ISH Fluorescein RNA probe |
This lab |
N/A |
| VECTASHIELD antifade mounting medium |
Vector Laboratories |
Cat# H-1000 |
| DAPI |
Sigma-Aldrich |
Cat# 9542 |
| NBT/BCIP Stock Solution |
Roche |
Cat# 11681451001 |
| Aqua-Poly/Mount |
Polysciences |
Cat# 18606–20 |
| Deposited Data |
| Raw data for smFISH |
This paper |
Zenodo Data https://doi.org/10.5281/zenodo.3258890
|
| Experimental Models: Drosophila Strains |
| y[1] w[1118] |
Bloomington Drosophila Stock Center |
Cat# 6598 |
| ΦC31 (y+); 38F1 (w+) |
This lab |
|
| y[1] M{vas-Cas9.RFP-}ZH-2A w[1118] |
Bloomington Drosophila Stock Center |
Cat# 55821 |
| y[1]w[67c23] P{y[+mDint2] = Crey}1b; D[*]/TM3, Sb[1] |
Bloomington Drosophila Stock Center |
Cat# 851 |
| hb14F/Tm3B, Sb, hb-lacZ
|
Christine Rushlow’s lab |
N/A |
| Flies with transgene at 38F1 landing site on chromosome II |
This paper |
N/A |
| ΔP2C |
This paper |
N/A |
| ΔP1C |
This paper |
N/A |
| ΔProx |
This paper |
N/A |
| ΔDist |
This paper |
N/A |
| ΔProx, ΔP2 Zld |
This paper |
N/A |
| Oligonucleotides |
| ΔP2C gRNA 1: GTTATATATCGCTCAGGTAGA |
This paper |
N/A |
| ΔP2C gRNA 2: GATTTGTCGGGATTTTCGTA |
This paper |
N/A |
| ΔP1C gRNA 1: GTTGGCGAGTGCACTGTTGTG |
This paper |
N/A |
| ΔP1C gRNA 2: GCGTTTCGTCCTTTGGATGTT |
This paper |
N/A |
| ΔProx/ΔProx ΔP2Zld gRNA 1: GAGGAGTAGGCAGCTAGCGT |
This paper |
N/A |
| ΔProx gRNA 2: GGTAGACGGATGCACGCGTCA |
This paper |
N/A |
| ΔProx ΔP2Zld gRNA 2: GTTATATATCGCTCAGGTAGA |
This paper |
N/A |
| ΔDist gRNA 1: GTCTCAACAGAAGTTCCTTCG |
This paper |
N/A |
| ΔDist gRNA 2: GCCCAAATATTCCACATAAAC Recombinant DNA |
This paper |
N/A |
| Prox-P2-lacZ |
This paper |
N/A |
| Dist-P2-lacZ |
This paper |
N/A |
| Prox-P1-lacZ |
This paper |
N/A |
| Dist-P1-lacZ |
This paper |
N/A |
| Dist short-P1-lacZ |
This paper |
N/A |
| 5.6 kb hb dual reporter wt |
This paper |
N/A |
| 5.6 kb hb dual reporter ΔP2 |
This paper |
N/A |
| 5.6 kb hb dual reporter P2L at P1 |
This paper |
N/A |
| 5.6 kb hb dual reporter P2C at P1 |
This paper |
N/A |
| 5.6 kb hb dual reporter P2C/2 at P1 |
This paper |
N/A |
| 5.6 kb hb dual reporter P1L at P2 |
This paper |
N/A |
| 5.6 kb hb dual reporter P1C at P2 |
This paper |
N/A |
| 5.6 kb hb dual reporter P2C ΔTATA at P1 |
This paper |
N/A |
| 5.6 kb hb dual reporter P2C ΔZld(S) at P1 |
This paper |
N/A |
| 5.6 kb hb dual reporter P1 + Zld(S) |
This paper |
N/A |
| 5.6 kb hb dual reporter P1 +change Zld (W->S) |
This paper |
N/A |
| 5.6 kb hb dual reporter P1 +Zld(S) + TATA |
This paper |
N/A |
| 5.6 kb hb dual reporter P1 change Zld (W->S) + TATA |
This paper |
N/A |
| 5.6 kb hb dual reporter P1+ Zld(S) + TATA + InrInr |
This paper |
N/A |
| 5.6 kb hb dual reporter P1+ Zld(W) + TATA + InrInr |
This paper |
N/A |
| 5.6 kb hb dual reporter P1+ Zld(S) + 10bp + TATA +InrInr |
This paper |
N/A |
| 5.6 kb hb dual reporter ΔProx |
|
N/A |
| 5.6 kb hb dual reporter ΔDist ΔProx |
This paper |
N/A |
| 5.6 kb hb dual reporter ΔDist |
This paper |
N/A |
| 5.6 kb hb dual reporter ΔP2Zld |
This paper |
N/A |
| 5.6 kb hb dual reporter ΔProx ΔP2Zld |
This paper |
N/A |
| pHD-DsRed-2XattP Software and Algorithms |
This paper |
N/A |
| MATLAB |
The Mathworks Inc. |
https://www.mathworks.com |
| FISH Toolbox Other |
Little et al., 2013 |
N/A |
| Confocal microscope |
Leica |
SP8 |
| Microscope |
Zeiss |
Axioskop |
| Digital camera for microscopy |
Zeiss |
AxioCam MRc |
