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. Author manuscript; available in PMC: 2019 Nov 27.
Published in final edited form as: Nat Mater. 2019 May 27;18(10):1124–1132. doi: 10.1038/s41563-019-0385-5

Figure 2. AuNP/CRISPR can deliver CRISPR components to the nucleus of HSPCs.

Figure 2.

a, Nucleus of primary human CD34+ HSPC following addition of AuNP/CRISPR to the culture (blue, Hoechst). b, Fluorophore tagged crRNA (green, Alexa488). c, Fluorophore tagged ssDNA (Red, Alexa660). Visible small vesicles on the far left side of the image suggest passive uptake by endocytosis. d, Overlay of all three stains showed colocalization of crRNA and ssDNA. a-d, Images are at 60× magnification. Data are representative of a single human donor different from all other donors reported in this study. Experiment was repeated once as an independent biological replicate with a similar result.