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. Author manuscript; available in PMC: 2019 Nov 27.
Published in final edited form as: Nat Mater. 2019 May 27;18(10):1124–1132. doi: 10.1038/s41563-019-0385-5

Figure 3. Optimization of HDR conditions and optimal editing dosage.

Figure 3.

a and b, Total editing and HDR levels. Data are means ± SEM (n=2 independent experiments). c, T7EI and NotI restriction enzyme digestions showing the related digestion bands. Experiment has been repeated once with similar results. d and e, Effect of different AuNP/CRISPR nanoformulation concentrations on total editing and HDR in primary human HSPC. Data are means ± SEM (n=3 independent experiments). f, Toxicity in primary human CD34+ cells as a function of concentration. Data are means ± SEM (n=3 independent experiments). Statistical significance was determined by a two-sided t-test. †: t value=28.58, degrees of freedom (df)=2, effect size (d)=70.79; ††: t value=37, df=2, d=56.11; †††: t value=28.58, df=2, d=36.73. Data are representative of a single human donor different from all other donors reported in this study.