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. Author manuscript; available in PMC: 2020 Mar 16.
Published in final edited form as: Nat Immunol. 2019 Sep 16;20(10):1335–1347. doi: 10.1038/s41590-019-0480-4

Figure 2: Deletion of Ptpn2 enhances formation of the Tim-3+ terminally exhausted subpopulation during LCMV Clone 13 infection.

Figure 2:

(a) Representative flow cytometry plots of Tim-3 and Slamf6 expression on control or Ptpn2-deleted P14 T cells in the spleen 8 days post LCMV Clone 13 infection. Representative of eight independent experiments, n ≥ 4 mice. (b) Ratio of Tim-3+/Slamf6+ control or Ptpn2-deleted P14 T cells in the spleen 8, 15, 22, and 30 days post LCMV Clone 13 infection. Representative of two independent experiments, n ≥ 4 mice. (c) Number of Tim-3+ control or Ptpn2-deleted P14 T cells in the spleen 8, 15, 22, and 30 days post LCMV Clone 13 infection. Representative of two independent experiments, n ≥ 4 mice. (d) Number of Slamf6+ control or Ptpn2-deleted P14 T cells in the spleen 8, 15, 22, and 30 days post LCMV Clone 13 infection. Representative of two independent experiments, n ≥ 4 mice. (e) Quantification of Granzyme B expression for Tim-3+ control or Ptpn2-deleted P14 T cells in the spleen 8, 15, 22, and 30 days post LCMV Clone 13 infection. Representative of two independent experiments, n ≥ 4 mice. (f) Quantification of BrdU incorporation for Tim-3+ control or Ptpn2-deleted P14 T cells in the spleen 8, 15, and 30 days post LCMV Clone 13 infection. Representative of two independent experiments, n ≥ 3 mice. (g) Quantification of Granzyme B expression for Slamf6+ control or Ptpn2-deleted P14 T cells in the spleen 8, 15, 22, and 30 days post LCMV Clone 13 infection. Representative of two independent experiments, n ≥ 4 mice. (h) Quantification of BrdU incorporation for Slamf6+ control or Ptpn2-deleted P14 T cells in the spleen 8, 15, and 30 days post LCMV Clone 13 infection. Representative of two independent experiments, n ≥ 3 mice. Bar graphs represent mean and error bars represent standard deviation. Statistical significance was assessed by two-sided Student’s paired t-test (b-h) (ns p>.05, * p≤.05, ** p≤.01, *** p≤.001, **** p≤.0001). See also Supplementary Figure 2.