Figure 4.

siRNA knockdown of USP18 enhances STAT activation and expression of ISGs in IFN‐β‐treated MDMs. MDMs from 6 donors were transfected with nontargeting (NT) control or USP18 siRNA for 3 h followed by IFN‐β treatment for 18 h. (A) Expression of p‐STAT1, p‐STAT2, and USP18 was measured by Western blot. (B) Expression of ISGs was measured by RT‐qPCR. A paired T‐test was used to compare control siRNA treated and USP18 siRNA treated samples that were untreated or IFN‐β treated. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant