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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Semin Cell Dev Biol. 2019 Mar 21;95:130–141. doi: 10.1016/j.semcdb.2019.02.003

Figure 2: Phylogenetic analysis of SV2 paralogs and SVOP.

Figure 2:

Shown is a dendrogram illustrating the relatedness of SV2, SVOP and homologous proteins in invertebrates. To generate the dendrogram, Human SV2A, SV2B, SV2C and SVOP protein sequences were used in a series of NCBI BLASTP queries. The best BLASTP hit (lowest E-value) per paralog for each species was taken as a representative sequence. Protein sequences were aligned using the sequence alignment tool MUSCLE [136]. Sequence alignment was further refined using MAFFT under the Li-NSI option [137]. The phylogenetic tree was generated using the Maximum Likelihood method as implemented in ETE 3 [138]. Schematic drawings of paralogs were generated using Protter [135]. Protein sequences used for tree construction were: synaptic vesicle glycoprotein 2A [Homo sapiens] (NP_001315603); synaptic vesicle glycoprotein 2A [Mus musculus] (NP_071313); synaptic vesicle glycoprotein 2A [Rattus norvegicus] (NP_476558); synaptic vesicle glycoprotein 2A [Danio rerio] (XP_696434); synaptic vesicle glycoprotein 2A [Xenopus tropicalis] (XP_012825002); synaptic vesicle glycoprotein 2B [Homo sapiens] (NP_001309966); synaptic vesicle glycoprotein 2B [Mus musculus] (NP_001347503); synaptic vesicle glycoprotein 2B [Rattus norvegicus] (NP_476555); synaptic vesicle glycoprotein 2B [Danio rerio] (XP_005161575); synaptic vesicle glycoprotein 2B [Xenopus tropicalis] (XP_004912713); synaptic vesicle glycoprotein 2C [Homo sapiens] (NP_055794); synaptic vesicle glycoprotein 2C [Mus musculus] (NP_083486); synaptic vesicle glycoprotein 2C [Rattus norvegicus] (NP_113781); synaptic vesicle glycoprotein 2C [Danio rerio] (NP_001121811); synaptic vesicle glycoprotein 2C [Xenopus tropicalis] (XP_002934873); synaptic vesicle glycoprotein 2C isoform X1 [Strongylocentrotus purpuratus] (XP_003723431); synaptic vesicle glycoprotein 2C-like [Aplysia californica] (XP_012946265); synaptic vesicle 2-related protein isoform X2 [Strongylocentrotus purpuratus] (XP_003726207); synaptic vesicle 2-related protein isoform X3 [Xenopus tropicalis] (XP_002932106); synaptic vesicle 2-related protein isoform 1 [Homo sapiens] (NP_061181); synaptic vesicle 2-related protein isoform 1 [Mus musculus] (NP_081081); synaptic vesicle 2-related protein isoform 1 [Rattus norvegicus] (NP_599231); synaptic vesicle 2-related protein [Danio rerio] (XP_005165254); CG3168, isoform A [Drosophila melanogaster] (NP_572345); CG4324, isoform A [Drosophila melanogaster] (NP_611868); Dmel_CG31272 [Drosophila melanogaster] (NP_650013); svop-1 [Caenorhabditis elegans] (NP_498960.2).