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. 2019 Sep 16;14:7549–7560. doi: 10.2147/IJN.S215087

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© 2019 Su et al.

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(A) After incubation with the CD44-SPIONPs, the labeled cells displayed the typical blue color, whereas unlabeled cells did not exhibit blue staining. (B) Location and internalization of targeted SPIONPs with Cal-27 cells and CSCs. The cell nucleus was stained with DAPI solution (blue). Location of CD44-SPIONPs after being in contact with CSCs for 24 h; the green fluorescence increased significantly. Arrows show CD44-SPIONPs in endocytic structures. Location of CD44-SPIONPs after being in contact with Cal-27 cells for 24 h; a few nanoparticles entered the cell membrane.