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. 2019 Sep 16;14:7533–7548. doi: 10.2147/IJN.S218057

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© 2019 Ding et al.

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Expression and purification of recombinant Cap-nM2e proteins.

Notes: (A) Primary pattern structure of recombinant Cap-nM2e. (B) Schematic illustration of Cap-3M2e VLPs nanovaccine. VLPs pattern was drawn from PDB accession number 3R0R. (C) SDS-PAGE analysis of Cap-nM2e protein expressed in E. coli BL21 (DE3). Lane M: molecular weight markers; Lane 1–7: SDS-PAGE analysis of total cell lysate of pET28a vector, Cap, Cap-M2e, Cap-2M2e, Cap-3M2e, Cap-4M2e and Cap-5M2e proteins expressed in E. coli BL21 (DE3). Red arrow indicates successfully expressed proteins. (D) SDS-PAGE analysis of purified Cap-nM2e protein. (E) Western blot analysis of purified Cap-nM2e protein using PCV2-specific polyclonal antibody. (F) Western blot analysis of purified Cap-nM2e protein using 14C2 mAb (Anti-IAV M2 protein). Lane M: molecular weight markers; Lane 1–4: SDS-PAGE analysis of purified Cap, Cap-M2e, Cap-2M2e, Cap-3M2e.

Abbreviations: Cap, Capsid; M2e, ectodomain of matrix protein 2; VLPs, virus-like particles; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; E. coli., Escherichia coli; PCV2, porcine circovirus type 2; mAb, monoclonal antibody.