Fig. 1.

A summary of the assay concept, displaying primers and different, fluorescently -labeled probes for the ITS2 region in genera Cooperia and Ostertagia. Primers “UnivF” and “UnivR” are displayed as light-gray half-arrows, whereas different probes—“Universal,” “Cooperia,” “Ostertagia” (and their sequences)—are depicted as dark gray rectangles with either FAM™ (blue circle) or HEX™ (green circle) fluorescent dyes attached to them. A single nucleotide difference, found between the consensus sequences of the ITS2 region in both genera of parasites, is highlighted in different shades of red between the two genera specific probes and their sequences