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. 2019 Sep 20;10(10):702. doi: 10.1038/s41419-019-1870-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Top: a 10 μM Cytochalasin D for 30 min; b 80 μM Dynasore for 30 min; c 1 mM Amiloride for 10 min; d 1 μg/ml Filipin for 30 min; and e drug-free medium; followed by incubation with 14 µM APT (30)-NPs for 2 h. Nuclear DNA was labeled with Hoechst 33342; Bottom: Mean fluorescence intensity (M.F.I) values of APT(30)-NPs in A549 cells incubated with different inhibitors were determined with IMARIS software for analysis of image data. The red fluorescence channel was defined between 10 and 100 for all images presented. Values presented are means ± SE.