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. 2019 Sep 20;9:13629. doi: 10.1038/s41598-019-49810-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profiles of candidate reference gene in Chilo partellus. (A) Developmental stage, (B) sex, (C) crude extracts of bacterially-expressed dsRNA, and (D) Purified dsRNA. Expression data are displayed as mean Ct values for each reference gene in all samples. Whiskers represent the maximum and minimum value while the box indicates the 25 and 75th percentiles and line across the box indicates the median.