Table 2.

Stability evaluation of candidate reference genes during feeding assay of C. partellus fed with purified and crude extracts of bacterially-expressed dsRNA over a period of 5 and 15 days using Normfinder, geNorm and BestKeeper algorithms and deltaCt algorithm.

Purified-dsRNA						Bacterially- Induced dsRNA				Developmental stages		Sex
Algorithm	Reference gene	5days of feeding		15 days of feeding		5 days of feeding		15 days of feeding
NormFinder		stability	Rank	stability	Rank	stability	Rank	stability	Rank	stability	Rank	stability	Rank
	Actin	0.408	4	0.562	5	0.877	4	1.188	7	2.37145	5	0.617	4
	18S	0.395	3	0.464	2	0.932	5	0.762	3	2.999018	6	3.92	6
	RPL32	0.808	6	0.529	3	1.072	7	0.87	6	1.955363	4	1.818	5
	V-ATPase	0.291	2	0.552	4	0.418	1	0.805	4	0.484509	2	0.379	3
	Tubulin	0.449	5	0.836	7	0.516	3	0.51	2	0.425675	1	0.149	1
	HSP70	0.977	7	0.807	6	1.534	6	0.827	5	1.736871	2	1.818	5
	ELF	0.177	1	0.33	1	0.481	2	0.453	1	1.738245	3	0.272	2
Bestkeeper	Actin	1.2	4	0.91	4	0.51	2	1.45	7	3.59	6	2.55	5
	18S	1.57	5	0.92	5	1.19	4	0.93	3	5.04	7	5.14	6
	RPL32	0.57	1	0.42	2	0.4	1	0.43	1	1.25	1	0.02	1
	V-ATPase	1.19	2	0.82	3	0.73	3	1.03	4	2.3	3	0.94	2
	Tubulin	1.16	3	1.08	6	1.32	6	1.08	6	2.96	4	1.24	3
	HSP70	1.98	6	1.17	7	2.39	7	1.06	5	2.9	5	0.02	1
	ELF	1.2	4	0.4	1	1.26	5	0.66	2	2.05	2	2	4
∆CT	Actin	0.94	3	1.15	4	1.69	4	1.93	7	3.76	6	2.64	4
	18S	0.97	4	1.08	2	1.8	5	1.58	5	5.01	7	5.69	7
	RPL32	1.35	6	1.12	3	1.84	6	1.66	6	3.63	5	2.82	5
	V-ATPase	0.86	2	1.17	5	1.46	1	1.53	3	2.63	2	2.14	2
	Tubulin	0.97	4	1.45	7	1.51	3	1.4	2	2.54	1	2.07	1
	HSP70	1.54	7	1.42	6	2.4	7	1.56	4	2.66	3	2.82	5
	ELF	0.8	1	1	1	1.5	2	1.31	1	3.46	4	2.25	3
Genorm	Actin	0.57	3	0.903	3	2.734	5	1.566	6	2.734	5	1.81	3
	18S	0.671	4	0.866	2	3.383	6	1.421	5	3.383	6	2.918	6
	RPL32	0.87	5	0.426	1	2.263	4	1.147	3	2.263	4	0.12	1
	V-ATPase	0.501	1	0.949	4	1.378	2	0.692	1	1.378	2	0.9	2
	Tubulin	0.501	1	1.199	6	0.92	1	1.308	4	0.92	1	1.116	4
	HSP70	1.062	6	1.1	5	0.92	1	0.692	1	0.92	1	0.12	1
	ELF	0.599	2	0.426	1	2.016	3	0.813	2	2.016	3	1.496	5