Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 20;10:4318. doi: 10.1038/s41467-019-12299-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Vectorial folding of “light-up” RNA aptamers. a Schematic of unfolding of an aptamer-heteroduplex via Rep-X and its subsequent folding (Spinach2 and iMangoIII are shown as representations here). b E histograms of heteroduplex in the Rep-X unbound (top) and after Rep-X unwinding in the presence of ATP (middle) and AMP-PNP (bottom). c Representative single-molecule trajectories (blue) showing co-transcriptional folding of an aptamer (Spinach2 (left), iMangoIII (middle), and MangoIV (right), 30 ms integration time). The red and black arrows indicate two-step RNA folding corresponding to formation of GQ (red) and the underlying base triple (black, iMangoIII) and duplex stem (black, MangoIV). Individual time trajectories have been synchronized (“Methods” section) and overlaid on to the representative traces. Population information is represented as: light yellow (lowest occupancy) to red (highest occupancy)