Table 1.
X-ray crystallographic data collection and refinement statistics for the X. oryzae yybP–ykoY Mn2+ riboswitch
| Xory yybP–ykoY riboswitch | Crystal 1 |
|---|---|
| Data collection | |
| Space group | P 21 21 21 |
| Cell dimensions | |
| a, b, c (Å) | 81.32, 85.19, 92.49 |
| α, β, γ (°) | 90, 90, 90 |
| Resolution (Å) | 62.66–2.96 (3.07–2.96)* |
| Rsym or Rmerge | 0.110 (1.51) |
| I /σI | 14.2 (1.26) |
| Completeness (%) | 99.83 (99.63) |
| Redundancy | 81.90 |
| CC1/2 | 1 (0.599) |
| CC* | 1 (0.866) |
| Refinement | |
| Resolution (Å) | 62.66–2.96 (3.07–2.96) |
| No. of reflections | 13901 (1361) |
| Rwork/Rfree | 0.205 (0.220) |
| No. of atoms | |
| RNA | 4214 |
| Ligand/ion | 120 |
| Water | 7 |
| B-factors | |
| RNA | 69.17 |
| Ligand/ion | 93.43 |
| Water | 66.69 |
| R.M.S. deviations | |
| Bond lengths (Å) | 0.005 |
| Bond angles (°) | 0.59 |
| PDB accession code | 6N2V |
Note that the data were mildly anisotropic, with reflections along thelreciprocal axis stronger. Thus, I/sigma for these reflections was well over 1.2 (indeed, over 1.5 up to 2.85 Å resolution)
*One crystal was used. Values in parentheses are for highest-resolution shell