Fig. 6.

Activation of effector and central memory CD4⁺ and CD8⁺ T cells after DENV infection. Activation (CD69⁺) of effector memory (T-EM: CD3⁺CD4⁺CD28⁻CD95⁺) and central memory (T-CM: CD3⁺CD4⁺CD28⁺CD95⁺) T cells within (a–c) CD4⁺ and (d–f) CD8⁺ T-cell compartments before and after DENV infection. Percent of cells were determined by immunophenotyping using flow cytometry (Supplementary Fig. 7 for gating strategy). Blue, orange, and black squares represent T-EM for ZIKVPF-10mo, ZIKVPR-2mo, and naive, respectively. Gray squares represent T-CM for each group. Short black lines mark mean value for each group per timepoint. Cutted line divide % of T-EM and T-CM cells quantified before and after DENV infection. Statistically significant differences within groups were determined using two-way ANOVA adjusted for Dunnett’s multiple comparisons test (comparison of each group response at each timepoint versus baseline of the same group) including two families, and seven comparisons per family. Significant differences are reported as multiplicity adjusted p-values (*< 0.05, **< 0.01, ***< 0.001, ****< 0.0001). Asterisks represent significant difference between the corresponded timepoint and baseline within the same group. Source data are provided as a Source Data file