Fig. 3. LPS induced the same immunological alteration of MM-MSC in healthy MSC.

a Neutrophils isolated from co-culture with HC-MSC pre-treated with LPS were able to inhibit T cell proliferation. b After the pre-treatment with LPS, HC-MSC educated-neutrophils showed pro-angiogenic capacity in vitro. 1: HMEC control; 2: HMEC in presence of VEGF-A (positive control); 3: HC-MSCed-N; 4: plus ed-N isolated from co-culture of PBMC with HC-MSC pre-treated with LPS. Magnification ×100. c MyD88 expression was increased in MM-MSC (n = 6) compared to HC-MSC (n = 3). For western blotting analysis, the optical density of the bands was measured using Scion Image software. All showed results represent the means of four independent experiments; error bars denote SD. *p < 0.05; ***p < 0.001