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. 2019 Sep 20;2:350. doi: 10.1038/s42003-019-0596-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Validation of proteomic assessment of expression of cell surface proteins by flow cytometry. a Variability of expression of RBC surface proteins from an independent set of UK donors assessed by flow cytometry. Fluorescent signal intensity is shown on the x-axis, and normalised count on the y-axis. Relative abundance of the same four surface proteins as determined by proteomics from the original set of nine UK donors is plotted as an adjacent box and whisker plot showing mean, median and interquartile ranges, normalised to the maximum for each sample. b Comparison of %CV values from proteomics with %CV of the median fluorescence intensity from flow cytometry for all four RBC surface proteins (n for flow cytometry = 7 or 8, n for proteomics = 9)