Fig. 2.

High affinity binding between OTULIN and SNX27. a Schematic representation of human OTULIN and SNX27 domain structure. b Sequence alignment and conservation of C-terminal PDZbm in OTULIN orthologues from tetrapod animals. c, d HEK293 cells were co-transfected with Flag-OTULIN and GFP-SNX27 constructs as indicated. Co-IPs were performed using anti-Flag antibody and tested for OTULIN and SNX27 interaction by WB. e ITC data for OTULIN PDZbm peptide (green) or OTULINcat (black) titrated against SNX27 PDZ domain. f ITC data for OTULINcat ΔETSL (red) and the reciprocal titration of OTULIN into SNX27 (purple). g Table summarizing the ITC data with mean parameters and the standard deviation derived from at least two independent experiments. Source data are provided as a Source Data file