Fig. 3.

Structure of the OTULIN-SNX27 complex revealing the canonical PDZ-PDZbm interface. a Structure of OTULIN (blue cartoon) bound to SNX27 (brown surface). The OTULIN catalytic center and also the OTULIN PDZbm are highlighted (dotted lines). b Surface representation of OTULIN colored based upon conservation calculated through the Consurf webserver. The binding site for Met1 diubiquitin is shown as cartoon from superimposition of the OTULINcat Met1 diubiquitin structure (PDB: 3ZNZ). c Close-up view of the interaction between OTULIN PDZbm and SNX27_PDZ rotated 90° from enclosed box in a. A weighted 2Fo-Fc map is shown for residues from the OTULIN PDZbm contoured at 1.1σ. Hydrogen bonds are denoted by dotted lines. d Additional residues from OTULIN PDZbm engage with SNX27. A canonical PDZbm cargo of SNX27 (DGKζ; PDB: 5ELQ) (green) is superimposed onto the OTULIN-SNX27 structure. Residues at each position are shown and numbered according to convention. e Mutation of the canonical binding site disrupts the interaction between OTULIN and SNX27. ITC data for either a single point mutation within the OTULIN PDZbm (black) or SNX27 PDZ (red) are shown