Skip to main content
. 2019 Sep 20;10(10):700. doi: 10.1038/s41419-019-1938-x

Fig. 5. RIPK1 kinase activity regulates cytokine production and inflammasome activation in xiap−/− macrophages.

Fig. 5

a WT, xiap−/−, xiap−/−tnf−/−, xiap−/−tnfr1−/− and xiap−/−tnfr2−/− BMDMs were stimulated with TNC-TNF. After 12 h supernatant was taken and assayed for IL-1β, IL-18 and IL-6. b BMDMs were treated for 2 h with TNC-TNF and/or Nec-1s and analysed for TNF RNA expression. c BMDMs were treated overnight with TNC-TNF and/or different dilutions of Nec-1s (+ = 1 μM, ++ = 5 μM, +++ = 10 μM) and supernatant was assayed for IL-1β, IL-18 and IL-6. d BMDMs of WT, xiap−/− and xiap−/−ripk1KD/KD were treated with TNC-TNF for 16 h and showed reduced IL-1β, IL-18 and IL-16 production upon loss of RIPK1 kinase activity. e Representative western blot of BMDMs treated for 16 h with TNC-TNF and/or Nec-1s. Data shown are mean ± SEM including n = 2–4 biological replicates. Experiments were repeated at least three times independently. Statistical significance was calculated using two-way ANOVA with *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.