Fig. 3.

miR-138 induced synaptic and cognitive impairments. A, B Two-month-old APP/PS1 mice were injected with miR-138 agomir (miR-138) or scrambled control (miR-NC) into the hippocampus and 5 days later were tested in the Morris water maze. The latency to reach the platform from days 1 to 5 (A), and the crossing time and distance in the target quadrant in the test experiment (day 7) were recorded (B), then the mice were sacrificed for Golgi staining. C, D The spine density and mushroom percentage (bar = 5 μm) (C) and the expression of the synapse markers Synapsin I (Syn I), PSD-93, and PSD-95 were assessed (D). *P < 0.05, **P < 0.01, ***P < 0.001 vs miR-NC group.