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. 2019 Jun 5;35(5):781–790. doi: 10.1007/s12264-019-00397-2

Fig. 1.

Fig. 1

Schematic of the experimental strategy. A Timeline of virus injection into the LDT for retrograde trans-synaptic tracing. B Schematic of cell-type specific circuit tracing. The AAV-CAG-Dio-TVA-eGFP virus (AAV2/9) and the AAV-CAG-Dio-RG (AAV2/9) virus are Cre-dependent. RV-EvnA-DsRed only infects TVA-expressing cells and spreads to the primary afferent cells with the help of the glycoprotein. C Ratios of all the input cell counts from the whole brain to starter cell counts in the LDT (n = 4, P = 0.106, mean ± SEM).