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. 2019 Sep 20;9:13657. doi: 10.1038/s41598-019-50136-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PAM- and PAL-treated glioblastoma cells with and without NAC. (a) Intracellular ROS generated in response to PAM and PAL. Image of U251SP cells. Scale bar represents 50 μm. DIC, differential interference contrast. (b) Intracellular ROS levels were evaluated by measuring fluorescent intensity of the CM-H₂DCFDA reagent. More than 50 cells were measured. Data are the mean ± SEM. **P < 0.01 versus control.