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. 2019 Apr 26;35(5):802–814. doi: 10.1007/s12264-019-00376-7

Fig. 5.

Fig. 5

CDK18 promotes OPC differentiation through activating Erk1/2 signaling. A, B Western blots (A) and analysis (B) of P-Erk1/2 and Erk1/2 protein levels in purified OPCs transduced with CDK18 OE virus or CDK18i virus and control virus. C, D Representative western blots (C) and statistical analysis (D) of the effect of the Erk1/2 inhibitor U0126 (10 nmol/L) on the expression of P-Erk1/2, Erk1/2, and MBP in purified OPCs transduced with overexpressing virus (CDK18-OE) and control viruses. E Representative images showing the effect of U0126 (10 nmol/L) on the expression of MBP (red) among purified OPCs transduced with CDK18 OE and control virus (green) (scale bar, 50 µm). F Statistical analysis of the percentage of MBP +/GFP + cells in each group. The OPCs were all cultured in differentiation medium for 72 h. Results are expressed as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test in B one-way ANOVA with Tukey’s post hoc test in D, F; n = 3 independent experiments.