Fig. 5.

The magnitude of fitness contributions of genes in P. syringae do not correlate well with their absolute level of expression (Upper) or fold-change of these genes in planta compared to that in hrp-inducing MM or KB (Lower). Epiphytic (Left) and apoplastic (Center) gene expression in B728a is a measure of fluorescence in microarrays (27). A comparison of B728a apoplastic gene fitness to expression of orthologs in the closely related strain DC3000, measured in A. thaliana, reveals a similar trend (Right). DC3000 gene expression is calculated as the number of reads per million (28). Fold-change in gene expression was calculated as a log₂ of the ratio of gene expression estimated from microarray fluorescence (B728a) or RNA-seq (DC3000) in planta relative to that in MM or in KB (27). The Pearson correlation coefficients are shown.