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. 2019 Sep 4;116(38):19083–19089. doi: 10.1073/pnas.1905054116

Fig. 2.

Fig. 2.

Majority of LN neutrophils are within tissues and lie outside blood and lymphatic vessels. (A) Confocal image of unstimulated LysM-GFP inguinal LN. (Scale bars, 300 μm [Inset 1, 40 μm; Inset 2, 15 μm]; Z stack, 10 μm.) (B) Confocal quantification of LN neutrophil location; data are from 3 mice. (C) Confocal image of inguinal LN neutrophil location. (Scale bars, 300 μm [Inset, 30 μm]; Z stack, 20 μm.) (D) Confocal image of inguinal LN showing neutrophils near SCS macrophages. (Scale bar, 30 μm; Z stack, 20 μm.) (E) Confocal image of inguinal LN showing neutrophil colocalization with DCs. (Scale bar, 40 μm; Z stack, 11 μm.) (F) Intravital imaging of unstimulated LysM-GFP popliteal LN; neutrophil movements are in green tracks. (Scale bar, 40 μm; Z stack, 60 μm.) (G) Mean speed of LN neutrophils; data are from 4 movies, each dot representing one cell track. Mean ± SEM is shown.