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. 2019 Sep 4;116(38):19083–19089. doi: 10.1073/pnas.1905054116

Fig. 4.

Fig. 4.

Neutrophils are present in human lymph nodes and express MHCII. (A and B) Quantification of neutrophils in human inguinal, mesenteric, and thoracic (tho) LNs with (A) representative plots and (B) % live neutrophils. (C) Confocal image of human thoracic LN neutrophils. (Scale bars, 20 μm; Z stack, 18 μm.) (D) Confocal image of human mesenteric LN neutrophils. (Scale bars, 40 μm; Z stack, 12 μm.) (E and F) MHCII expression on human LN neutrophils with (E) representative plots and (F) % MHCII+ neutrophils. Data are from 14 donors. (G and H) MHCII expression on unstimulated murine neutrophils with (G) representative plots and (H) % MHCII+ neutrophils; data are from 6 mice in 5 experiments; *P < 0.05, ***P < 0.001 vs. blood. Mean ± SEM is shown.