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. 2019 Sep 4;116(38):19083–19089. doi: 10.1073/pnas.1905054116

Fig. 5.

Fig. 5.

FcγR cross-linking by IC increases neutrophil MHCII expression and CD4 T cell activation ex vivo. (A and B) MHCII expression on human blood neutrophils following 1-h ex vivo stimulation with ATP, LPS, S. pneumoniae or OVAIC (generated with whole OVA), with (A) representative plots and (B) % MHCII+ neutrophils; data are from 3 donors in triplicate; ***P < 0.001 vs. unstimulated. (C) H2-Aa, H2-Ab1, Cd80, Cd86, and Cd40 mRNA expression, relative to Gapdh (2−ΔΔCT), in murine BM neutrophils following 2-h ex vivo stimulation with OVA or OVAIC; 1 of 2 experiments is shown, each dot representing a triplicate; ***P < 0.001 OVAIC vs. PBS. (D and E) Expression of (D) CD25 and (E) CD69 on OTII CD4 T cells following coculture with OVA- or OVAIC-stimulated neutrophils; 1 of 2 experiments is shown; **P < 0.01, ***P < 0.001 vs. PMN + OTII T control. Mean ± SEM is shown.