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. 2019 Sep 4;116(38):19187–19192. doi: 10.1073/pnas.1910886116

Fig. 2.

Fig. 2.

Identification of ATG2 gene and impairment of stomatal opening in other autophagy-related mutants. (A) Map-based cloning of the ATG2 gene. ATG2 was mapped to the 231 kbp region between BAC clones MCB22 and MLD14. Numbers indicate the recombination from a total of 154 chromosomes. (B) Genomic structure and mutation sites of ATG2 gene. Black rectangles indicate the protein-coding region. (C) Light-dependent stomatal opening in F1 progenies obtained from crosses between the atg2-5 and peup1-1 mutants. (D) Rescue of light-dependent stomatal opening by transformation of atg2-5 mutant with GFP-ATG2. (E and F) Impairment of light-dependent stomatal opening in intact leaves (E) and the epidermis (F) in other autophagy-related mutants. For C−E, stomatal conductance was measured as described in Fig. 1B. Data represent means ± SEM (n = 3). For F, epidermal strips were incubated as described in Fig. 1C. Data represent means ± SD (n = 75, pooled from triplicate experiments). Different letters indicate significant difference (ANOVA with Tukey’s test, P < 0.01).