Figure 2. The Psq^S Isoform Colocalizes with Pc and GAF at Active Enhancers in Kc167 Cells.

(A) Binding motif detected by multiple expectation maximization (EM) for motif elicitation designed to analyze ChIP-seq (MEME-ChIP) at Psq^S peaks.

(B) Percentage of Psq^S peaks that overlap GAGA motifs ranked by ChIP-seq signal intensity (blue). Regions upstream and downstream of Psq^S summits were tested for comparison (gray).

(C) Example region showing Psq^S binding sites as peaks with a Psq^tot signal (blue), without a Psq^L signal (green), and colocalizing with GAF, Pc, CBP, and H3K27ac (black).

(D) Heatmap showing ChIP-seq signal for various proteins or histone modifications surrounding Psq^S binding sites ± 2 kb. n = 6,386. The STARR-seq signal is from S2 cells, and the ChIP-seq signal is from Kc167 cells and is shown relative to IgG.

(E) IGV track showing an example locus with ChIP-seq signal for Psq^L and Psq^tot overlapping with both Su(Hw) and GAF.

(F) ChIP-seq signal for various proteins and histone modifications in a 2-kb region surrounding sites enriched in Psq^L and Psq^tot. n = 2,130. The STARR-seq signal is from S2 cells, and the ChIP-seq signal is from Kc167 cells and is shown relative to IgG.

See also Figure S2.