Figure 5. Psq^S-Bound Enhancer-Promoter Interactions Are Altered during the Ecdysone Response.

(A) Pie graph showing the relative binding of Psq^S (purple), Psq^L (green), and Psq^L&S (pink) at enhancer-promoter interaction anchors determined by H3K27ac HiChIP in Kc167 cells. n = 94,483 interactions.

(B) 2D metaplot of Psq HiChIP signal around enhancer-promoter interactions determined by H3K27ac HiChIP found in (A). En, enhancers; Prm, promoters.

(C) Number of STARR-seq ecdysone enhancers (left) compared with random loci (right) that overlap Psq^S (purple), Psq^L (green), and Psq^L&S (pink).

(D) Psq HiChIP data in a 1-Mb region of chromosome 2R showing changes in the interaction profile after ecdysone treatment (bottom left) compared with the control (top right). ChIP-seq tracks showing Psq^S and EcR before (CTL) and after 20-HE treatment. The STARR-seq signal after ecdysone treatment is also shown.

(E) 2D metaplot of Psq HiChIP data before (top right) and after (bottom left) ecdysone treatment. Regions between STARR-seq ecdysone enhancers and nearby differentially expressed genes were scaled to equally sized bins. n = 86.

See also Figure S5.