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. Author manuscript; available in PMC: 2020 Feb 26.
Published in final edited form as: Nat Immunol. 2019 Aug 26;20(10):1372–1380. doi: 10.1038/s41590-019-0471-5

Fig. 2. Selective deployment of RUNX paralogs enables signal-responsive Langerhans cell differentiation.

Fig. 2

a) Expression of FLAG-RUNX by immature DC transduced with FLAG-RUNX1- or -3-IRES-GFP and sorted into GFP-low, intermediate, and high (lo/int/hi). Levels of retrovirally encoded RUNX3 protein were comparable to endogenous RUNX3 protein in wild-type immature DC (Supplementary Fig. 1a, right). Lamin is the loading control. Representative of 3 independent experiments.

b) Spi1-deficient BM cells were cultured with GM-CSF and TGF-β, and transduced with RUNX-IRES-GFP or IRES-GFP control vector after 24h. Numbers indicate percentages of CD11c+ MHC class II+ DEC205+ Epcam+ LH cells after 72h. Right: mean ± SD of 3 independent experiments. See Supplementary Fig. 1b for numbers of cells recovered. * P<0.05, ** P<0.01, *** P<0.001 by two-tailed T test between Runx1 and -3 (black), Runx1 and control vector (blue), Runx3 and control vector (red).

c) Spi1-deficient immature DC were cultured without TGFβ and analysed as in b. Right: mean ± SD of 3 independent experiments. Statistics as in b.