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. Author manuscript; available in PMC: 2020 Feb 26.
Published in final edited form as: Nat Immunol. 2019 Aug 26;20(10):1372–1380. doi: 10.1038/s41590-019-0471-5

Fig. 3. Selective deployment of RUNX paralogs enables signal-responsive induction of the Treg cell signature transcription factor Foxp3.

Fig. 3

a) Intracellular staining (left) shows rescue of Foxp3 induction in Runx1-deficient naive CD4 T cells at equivalent expression of RUNX1 and -3 (right). Mean ± SD of 3 independent experiments. Levels of retrovirally encoded RUNX1 protein were comparable to endogenous RUNX1 in wild-type CD4 T cells (Supplementary Fig. 2a). See Supplementary Fig. 2b for numbers of cells recovered from this and subsequent T cell experiments. * P<0.05, ** P<0.01, *** P<0.001 by two-tailed T test between Runx1 and -3 (black), Runx1 and control vector (blue), Runx3 and control vector (red).

b) Runx1-wild type naive CD4 T cells were activated for 18h, transduced with RUNX1- or -3-IRES-GFP or control vector and cultured without TCR stimulation, without TGF-β or PI3K/mTOR inhibitors. Mean ± SD of 3 independent experiments. Statistics as in a.