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. Author manuscript; available in PMC: 2019 Dec 4.
Published in final edited form as: Nat Microbiol. 2019 Jun 3;4(10):1760–1769. doi: 10.1038/s41564-019-0464-z

Figure 1. Genome-wide CRISPR-Cas9-mediated screen identifies host factors for TcdA.

Figure 1.

a. Schematic illustration of the screen process utilizing TcdA1–1874 on HeLa cells. Round zero (R0) represents cells at the beginning of the screen. Round 1, 2, 3 (R1, R2, and R3) represent surviving cells after exposure to TcdA1–1874 sequentially at indicated toxin concentrations. NGS: next-generation sequencing.

b. Genes identified after R3 were ranked and plotted. The y axis is the number of unique sgRNA for each gene. The x axis represents the number of sgRNA reads for each gene. The top-ranking genes are color-coded and grouped based on their functions.

c. The NGS reads from R0 to R3 for the top-20 ranked (ordered by NGS reads) genes in R3 were color-coded and plotted. The diameter of the circle represents the number of unique sgRNA detected for the gene. All these top-20 ranked genes progressively enriched from R0 to R3.