Table 4.
Mean positive signal concentration (DNA copies/unit) | ||||||||
---|---|---|---|---|---|---|---|---|
EC23S857 | Entero1 | GenBac3 | HF183 | Gull4 | Camp2 | Faecalis1 | Faecium1 | |
Gull feces (1 g) | ||||||||
aGHCB to CL & GHCB to MWMb | 1.8 × 103 | 6.5 × 104 | 6.5 × 102 | < LOQ | 8.9 × 107 | 3.8 × 106 | < LOQ | < LOQ |
Gull cloacae (1 ml) | ||||||||
GHCB to CL & GHCB to MWMb | 2.0 × 103 | 1.1 × 104 | 1.8 × 102 | 6.3 × 101 | 7.0 × 103 | < LOQ | < LOQ | < LOQ |
GHCB to CL | 8.3 × 102 | 4.5 × 103 | < LOQ | < LOQ | 2.3 × 105 | 1.2 × 106 | < LOQ | < LOQ |
GHCB to MWM & GHCB to ML | 9.7 × 102 | 4.2 × 104 | < LOQ | < LOQ | 3.4 × 106 | 3.7 × 104 | < LOQ | < LOQ |
GHCB to CL | 1.3 × 103 | 2.9 × 104 | < LOQ | 5.8 × 101 | 9.0 × 105 | < LOQ | 6.7 × 104 | < LOQ |
GHCB to CL | 1.3 × 103 | 7.4 × 105 | < LOQ | < LOQ | 4.0 × 104 | 8.8 × 104 | 3.2 × 106 | < LOQ |
< LOQ, below limit of quantification. LOQs and range of amplification efficiency for each qPCR are noted in Table S1.
Flight path, gulls were tracked flying from site A to B using radio telemetry technology where GHCB to CL is Grand Haven City Beach to Coopersville Landfill; GHCB to MWM is Grand Haven City Beach to Muskegon Wastewater Management lagoon; GHCB to ML is Grand Haven City Beach to Muskegon Landfill.
Feces and cloacae samples were collected from the same gull.