Fig. 5.

Nrf2 immunofluorescence. HT22 cells were cultured in normal glucose medium (25 mM d-glucose), high-glucose medium (50 mM d-glucose), high glucose-medium + 1.25 µm/L SFN, high-glucose medium + 2.5 µm/L SFN or high-glucose medium + 5 µm/L SFN for 0 or 72 h. Nrf2 fluorescence (Cy3) was measured by using a laser scanning confocal microscope at a magnification of ×200, and the excitation/emission wavelength was 488/532 nm. The fluorescence intensity in the nucleus was decreased in high-glucose medium but significantly increased in high-glucose medium with different concentrations of SFN after culturing for 72 h