Table 1.
Primers used for the quantitative real-time PCR.
| Primers | GenBank number | Sequence (5′-3′) | Size (bp) |
|---|---|---|---|
| β-Actin | NM_007393.5 | F: CGTTGACATCCGTAAAGACC | 111 |
| R: CTAGGAGCCAGAGCAGTAATC | |||
| MCP-1 | NM_011333.3 | F: CCCTAAGGTCTTCAGCACCT | 118 |
| R: ACTGTCACATGGTCACTCC | |||
| TNF-α | NM_013693.3 | F: GTGCCTATGTCTCAGCCTCT | 129 |
| R: CTGATGAGAGGGAGGCCATT | |||
| IL-6 | NM_031168 | F: AGACTTCCATCCAGTTGCCT | 113 |
| R: CAGGTCTGTTGGGAGTGGTA | |||
| IGF-I | NM_010512.5 | F: ACTGGAGATGTACTGTGCCC | 105 |
| R: GATAGGGACGGGGACTTCTG | |||
| IGF-IR | NM_010513 | F: TCCCCAACCTCACAGTCATC | 146 |
| R: GTCGGCGTTCTTCTCAATCC | |||
| IGFBP-5 | NM_010518 | F: CGAGATGGCTGAAGAGACCT | 112 |
| R: CTGGGTCAGCTTCTTTCTGC |
MCP-1: monocyte chemoattractant protein-1; TNF-α: tumor necrosis factor-α; IL-6: interleukin-6; IGF-I: insulin-like growth factor-I; IGF-IR: insulin-like growth factor-I receptor; IGFBP-5: insulin-like growth factor binding protein-5.