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CMA formation in different types of collagen (I, II, III, and IV) and heat-denatured type I collagen. Four types of collagen (I, II, III, and IV) and heat-denatured type I collagen (denatured by heating at 60°C for 15 min) were incubated with 1 mM glyoxal at 37°C for up to 7 days, and the CMA content was determined by ELISA.
