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Silencing PINK1 inhibited Sal-induced activation of mitophagy in MN9D cells. (a) Mitochondrial protein expression of Parkin, LC3, p62, and LAMP2A in MN9D cells. VDAC1 served as loading controls. Qualification analysis of protein expression of Parkin (b), ratio of LC3II/LC3I (c), p62 (d), and LAMP2A (e) in the mitochondria. Each column represents the mean ± SD (n = 3). ∗P < 0.05 and ∗∗P < 0.01, compared with the control group; #P < 0.05 and ##P < 0.01, compared with MPP+-treated group.
