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. 2019 Sep 9;2019:1247276. doi: 10.1155/2019/1247276

Figure 8.

Figure 8

Wnt1 overexpression activated the Wnt/β-catenin pathway in U251 cells. U251 cells, U251-Wnt1 cells, or U251-EV cells were treated with vehicle, 6-OHDA (50 μM), or/and DKK-1 (100 ng/ml) for 24 h, and the protein levels of β-catenin (a) were detected by Western blot with β-actin as an internal control. The relative band intensities of β-catenin (b) were measured by Quantity One software and normalized to the expression of β-actin. Differences among groups were examined using ANOVA followed by using Tukey-Kramer tests for post hoc multiple comparisons. Data are presented as the mean ± SD of 3 experiments. F value = 30.23, dfbetween groups = 4, dfwithin groups = 10, and dftotal = 14.