Table 1. Gradient of mobile phases.
| Time (min) | A (%) |
B (%) |
Flow (mL/min) |
|---|---|---|---|
| 0 | 15 | 85 | 0.40 |
| 1 | 15 | 85 | 0.40 |
| 8 | 0 | 100 | 0.40 |
| 9 | 0 | 100 | 0.50 |
| 9.1 | 0 | 100 | 0.60 |
| 11.5 | 0 | 100 | 0.60 |
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