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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Nat Microbiol. 2019 Jul 8;4(10):1716–1726. doi: 10.1038/s41564-019-0497-3

Figure 2. The glycolysis-mediated cell death is suppressed under gluconeogenic conditions.

Figure 2

a, Effects of repression of sugar uptake system, PTS, on L-form growth. B. subtilis strains YK1601 (Pxyl-murE Pspac-ptsHI), YK1602 (Pxyl-murE ΔptsG), YK2124 (Pxyl-murE ΔsacP), YK2125 (Pxyl-murE ΔfruA) and YK2126 (Pxyl-murE ΔlevD) were streaked on NA/MSM (sucrose) plates with or without 1 mM IPTG, and in the presence (MurE ON) or absence (MurE OFF) of 1% xylose, and incubated at 30°C. No effects were observed in the walled cell growth in the presence of IPTG and xylose.

b, Phase contrast micrographs of YK1601 were taken from the plates without IPTG and xylose shown in panel a.

c, Effects of additional carbon sources on L-form growth on a complex rich medium (NA). B. subtilis strains BS115 (Pxyl-murE ispA+) and LR2 (Pxyl-murE ispA-) were streaked on NA plates with 0.5 M various carbon sources (sucrose, glucose, malate or succinate) in the absence of xylose (MurE OFF), and incubated at 30°C.

d, Phase contrast micrographs of BS115 or LR2 were taken from the plates shown in panel c.

The figures are representative of at least three independent experiments (a-d).